Molecular cloning and characterization of the pseudorabies virus UL31 gene.

Identification of Synonymous Codon Usage Bias in the Pseudorabies Virus UL31 Gene

Background: Little knowledge of synonymous codon usage pattern of pseudorabies virus (PRV) genome, especially the UL31 gene in the process for its evolution is available. Objectives: In the present study, the codon usage bias between PRV UL31 sequence and the UL31-like sequences was identified. Materials and Methods: We used a comprehensive analysi...

The interacting UL31 and UL34 gene products of pseudorabies virus are involved in egress from the host-cell nucleus and represent components of primary enveloped but not mature virions.

A 2.6-kbp fragment of the pseudorabies virus (PrV) genome was sequenced and shown to contain the homologues of the highly conserved herpesvirus genes UL31 and UL32. By use of a monospecific antiserum, the UL31 gene product was identified as a nuclear protein with an apparent molecular mass of 29 kDa. For functional analysis, UL31 was deleted by mutagenesis in Escherichia coli of an infectious f...

Detection, Cloning, Molecular Characterization and Phylogenic Analyses of a New Primate T-Cell Lymphotropic Virus Type I in Olive Baboon

Molecular Cloning of VPI gene of Foot-and-Mouth Disease Virus Type 01/Iran

Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate

White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...